Part:BBa_K3944033:Design
ScGAL1 + Z3BS-FatB
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1491
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 169
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 915
Illegal BsaI.rc site found at 1285
Illegal BsaI.rc site found at 1758
Illegal SapI site found at 734
Design Notes
May be assembled with scars in a biobrick compatible manner. Seven Z3BS repeats ensures optimal binding affinity. Z3BS repeats + ScGAL1 obtained as a single unit and cloned/assembled as such. FatB sequence was optimized for use in S.Cerevisiae.
Source
Assembled from parts obtained from IDT and systems biology at Chalmers technical highschool.
References
1: Joaquı́n J Salas, John B Ohlrogge,
Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases,
Archives of Biochemistry and Biophysics,
Volume 403, Issue 1,
2002,
Pages 25-34,
ISSN 0003-9861,
https://doi.org/10.1016/S0003-9861(02)00017-6.
2: Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae
R. Scott McIsaac, Sanford J. Silverman, Megan N. McClean, Patrick A. Gibney, Joanna Macinskas, Mark J. Hickman, Allegra A. Petti, and David Botstein
Molecular Biology of the Cell 2011 22:22, 4447-4459
3: R. Scott McIsaac, Patrick A. Gibney, Sunil S. Chandran, Kirsten R. Benjamin, David Botstein, Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae, Nucleic Acids Research, Volume 42, Issue 6, 1 April 2014, Page e48, https://doi.org/10.1093/nar/gkt1402